The Transmission Electron Microscope (TEM) has been used in all areas of biological and biomedical investigations because of its ability to image the finest cell structures. It is also used as a diagnostic tool in clinical pathology laboratories.
The Electron Microscopy Laboratory (EM Lab) at the Department of Pathology and Laboratory Medicine, Drexel University College of Medicine, is focused on diagnosis, education, and research with emphasis on diagnosis of renal diseases, muscular and peripheral nerve diseases, as well as cancer. It is a full-service laboratory equipped with state-of-the-art instrumentation for the preparation and analysis of biological specimens. It features a JEOL Transmission Electron Microscope, Model JEM 1230. The JEM 1230 is a high performance high contrast TEM with excellent imaging capabilities. It is operated at 40-120 kV and utilizes magnifications from 50x to 600,000x and resolution of 0.36 nm. In addition to the standard fluorescent viewing screen, it is equipped with 16 megapixel digital camera and software for both specimen viewing and image recording. The specimen preparation equipment includes state-of-the-art Leica UC6 ultramicrotome, capable of producing high quality semi-thin (thick) plastic sections and fine thin sections. In addition to fixed tissue, the laboratory can use paraffin blocks to prepare EM specimens.
The Electron Microscopy Lab is certified by CLIA and the Pennsylvania Department of Health. The laboratory adheres to the regulations set forth by CLIA and the Pennsylvania Department of Health in order to assay clinical samples, train residents, fellows and medical students, as well as conduct research. The laboratory features a climate of collegial interactions, making it mutually beneficial for all participants.
The Electron Microscopy Lab serves as a rotation site for residents and fellows to learn the basic electron microscopy techniques used in patient diagnostics. Residents and fellows become familiar with the sample preparation, TEM operation, specimen viewing and photographing, as well as image interpretation and analysis. In addition, the EM Lab is an educational site for the Master of Science Pathologist's Assistant program run by Drexel University.
In addition to its educational function, the EM lab has resources available to professors and students to aid their research. It offers specimen preparation of tissues and cells, as well as negative staining of macromolecules, viruses, and nanoparticles. Users of the EM Lab have the option of working with a staff member or they may take advantage of our comprehensive user training in order to work independently on the TEM. Please feel free to contact us with any questions or comments about our facility.